Search results for "flashing light"
showing 3 items of 3 documents
Technologies for cultivation and exploitation of microalgae in industrial applications
2020
This thesis work is focused on technologies for the development of the microalgal industry. During this 3-years PhD work several themes were addressed. Section 1 consists in a literature review concerning the general features of microalgae, their composition and a description of the photosynthetic process. Furthermore, themes such as biosynthetic pathways, growth kinetics, microalgae cultivation and their applications are reviewed. Section 2 focuses on the genetic manipulation of microalgae aimed at producing a vitamin B12-enriched food supplement. In this case, the human intrinsic factor gene, was inserted in the genome of the microalgae Chlamydomonas reinardhtii in order to integrate new …
Combined effect of nutrient and flashing light frequency for a biochemical composition shift in Nannochloropsis gaditana grown in a quasi-isoactinic …
2020
Artificial lighting may be an interesting opportunity for the cultivation of microalgae as an alternative to natural sunlight. In particular, light emitting diodes (LEDs) can be employed to tailor the lighting to the microalgal culture in a controlled mode in order to create flashing light. In order to establish the effect of the flashing frequency on growth and biochemical composition of a model microalga, a quasi-isoactinic reactor, in which the light distribution is almost homogeneous, was set up. In this work, it was employed for the cultivation of the heterokont Nannochloropsis gaditana in two growth media with limiting and nonlimiting nutrients. The combined effect of nutrient concent…
Flashing lights affect the photophysiology and expression of carotenoid and lipid synthesis genes in Nannochloropsis gaditana
2022
Nannochloropsis gaditana is a promising microalga for biotechnology. One of the strategies to stimulate its full potential in metabolite production is exposure to flashing lights. Here, we report how N. gaditana adapts to different flashing light regimes (5, 50, and 500 Hz) by changing its cellular physiology and the relative expression of genes related to critical cellular functions. We analyzed the differential mRNA abundance of genes related to photosynthesis, nitrogen assimilation and biosynthesis of chlorophyll, carotenoids, lipids, fatty acids and starch. Analysis of photosynthetic efficiency and high mRNA abundance of photoprotection genes supported the inference that excess excitati…